Breaking News: European Association of Urology Awards Institute for Prostate Cancer First Place Award
The European Association of Urology is holding its annual meeting in Paris this week. Our team has won first prize for best oncology abstract for the work presented in “Real-time, in vivo multiphoton microscopy imaging is a promising tool to identify prostate tissue during rat survival surgery.” This work was presented by Matthieu Durand who spent a year working at Cornell in New York. The full abstract can be found here and is reproduced below.
Multiphoton microscopy is a novel imaging modality that can differentiate various structures while performing prostate cancer surgery. This technology has the potential to reduce morbidity and complications during robotic prostate surgery.
Real-time, in vivo multiphoton microscopy imaging is a promising tool to identify prostate tissue during rat survival surgery
Durand, M.1, Aggarwal, A.2, Robinson, B.3, Srivastava, A.4, Sooriakumaran, P.4, Mtui, J.4, Brooks, D.4, Flomenbaum, D.4, Sterling, J.2, Mukherjee, S.2, Leung, R.4, Tewari, A.K.4
1Academic Hospital of Nice, Dept. of Urology, Nice, France, 2Weill Medical College of Cornell University, Dept. of Biochemistry, New York, United States of America, 3Weill Medical College of Cornell University, Dept. of Surgical Pathology, New York, United States of America, 4Weill Medical College of Cornell University, Dept. of Urology, New York, United States of America
Introduction & Objectives
Extraprostatic extension of prostate cancer is a microscopic phenomenon; therefore, it is not possible to differentiate cancerous cells from nerves during surgery. This can result in the incomplete removal of the cancer and/or postoperative impotence due to damage or excision of the nerves. In vivo multiphoton microscopy (MPM) is a novel technology that has shown promise in visualizing prostatic structures and adjacent nerves in an ex vivo setting. We investigated its role in an animal model, to discover whether it could be used in an in vivo setting.
Material & Methods
We used a custom-built MPM, consisting of an Olympus BX61WI upright frame and a modified Bio-Rad MRC 1024 scanhead. We used Sprague Dawley rat models, ex vivo and then in vivo for in-situ MPM imaging to assess its ability for identifying the anatomy of the prostatic tissue; we also examined the imaged tissue for signs of phototoxicity. A cocktail of ketamine and xylazyne was used as general anesthesia to keep the rat asleep and decrease body movement during imaging. Open surgical exposure of the prostate was performed to image the right prostatic lobe and the left lobe was used as an internal control. After image acquisition, the rat’s abdominal cavity was closed and the rat removed from the stage back to the husbandry unit for recovery. The rat was monitored for at least 15 days to check for signs of distress. Finally, the rat was euthanized and the whole prostate was processed for histological analysis to look for phototoxicity effects and to provide correlation between MPM ‘virtual’ histology and the gold standard.
MPM was correctly able to identify anatomical structures of the rat prostatic tissue, as seen in figure 1. No tissue damage was observed either during imaging, or in the histopathology slides prepared from the imaged specimens.
Real-time, MPM imaging is feasible and safe without damaging tissue structures or being life-threatening in rat models. This novel technology may in the future be translated to human subjects via intra-operative use of a multiphoton endoscope currently being developed by our collaborators.